By H. Mori, N. Nakagawa, T. Ono, N. Yamagishi, H. Imaseki (auth.), Jean Claude Pech, Alain Latché, Claudine Balagué (eds.)
ISBN-10: 9048142490
ISBN-13: 9789048142491
ISBN-10: 9401710031
ISBN-13: 9789401710039
The overseas Symposium on "Cellular and Molecular points of Biosynthesis and motion of the Plant Hormone Ethylenc" ,vas held in Agen, France from August 31 st and September 4th, 1992. The making plans and administration of the medical and social programme of the convention have been conducted together by way of the "Ethylene learn team" of ENSAlIN"P (Toulouse) and Agropole Congres carrier (Agen). because the final conferences in Israel (1984) and in Belgium (1988), ethylene body structure has undergone a interval of fascinating development because of new advancements in mobile and molecular bioiogy. New equipment and instruments were built to raised comprehend the position and services of ethylene in fruit ripening, flower senescence, abscission, piant progress, and phone differentiation. Genes fascinated with ethylene biosynthesis were characterised and transgenic crops with altered ethylene creation were generated. The feasibility of delaying fruit ripening or flower senescence through genetic manipulation is now verified, hence beginning new views for the postharvest dealing with of plant items. a few development has additionally been made at the knowing of ethylene motion. even if, a lot continues to be performed during this region to explain the ethylene sign transduction pathway. round a hundred and forty scientists from 20 nations attended the Symposium. They offered forty seven oral experiences and forty poster demonstrations. them all are released in those lawsuits. it's been a excitement for us to prepare this significant Symposium and to edit this book.
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Additional resources for Cellular and Molecular Aspects of the Plant Hormone Ethylene: Proceedings of the International Symposium on Cellular and Molecular Aspects of Biosynthesis and Action of the Plant Hormone Ethylene, Agen, France, August 31–September 4, 1992
Sample text
UJ 5 10 15 [NaOHllmMI 20 ~ UJ 00~-~,~0-~20~~~t~~60~ [NaHCn;llmMI Figure 3. Effect of NaOH addition on the EFE activity. An enzyme extract (900 JLI containing 473 JLI protein) was assayed either before or after passage through Sephadex. Figure 4. Effect of N aH C03 on the EFE activity. An ~nzyme extract (873 JLl) was assayed either directly (528 JLg protein), or after passage through Sephadex (349 JLg protein). 3 ACTIVATION BY BICARBONATE/CARBON DIOXIDE Activity of the desalted extract could also be restored by adding HCO;.
SMITH AND P. JOHN Department of Agricultural Botany Plant Science Laboratories University of Reading Reading RG6 2AS UK ABSTRACT. Activity of the ethylene-forming enzyme extracted from melon can be stabilised by the inclusion of 30% glycerol in media, so that there is virtually no los~ of activity when the enzyme is stored at O·C for 24 hr. When enzyme preparations from melon are desalted by passage through a column of Sephadex, activity is reduced. The lost activity can be restored by the inclusion of dilute NaOH to the reaction mixture, which gives a 6-fold stimulation of the EFE activity from melon.
The duration of C2H 4 treatment required to reverse the antisense phenotype is 6 days. c +-' W 50 60 70 Days after Pollination Figure 1. RNA (12). Inhibition of ethylene production in detached tomato fruits by antisense ACC synthase with C2H 4 do not develop a fully ripe phenotype compared with control fruits treated similarly. 2 ACC Deaminase Klee et al. (9) used a different approach to inhibit ethylene production. They overexpressed the ACC deaminase gene from Pseudomonas sp. in transgenic tomato plants which metabolizes ACC to a-ketobutyrate (9).
Cellular and Molecular Aspects of the Plant Hormone Ethylene: Proceedings of the International Symposium on Cellular and Molecular Aspects of Biosynthesis and Action of the Plant Hormone Ethylene, Agen, France, August 31–September 4, 1992 by H. Mori, N. Nakagawa, T. Ono, N. Yamagishi, H. Imaseki (auth.), Jean Claude Pech, Alain Latché, Claudine Balagué (eds.)
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